Detection of the separated compounds was performed densitometrically in absorption/reflectance mode at 220 nm and after exposure to iodine also by image analysis. Plates were developed in modified horizontal developing chamber using ethyl acetate–acetone–acetic acid–water (4:1:0.25:0.5, v/v). For the use of UTLC plates technical modifications of the commercially available equipments for the sample application, development and detection were made. The separation of structurally related angiotensin-converting enzyme (ACE) inhibitors lisinopril, cilazapril, ramipril and quinapril and their corresponding active diacid forms (prilates) by conventional TLC silica gel 60 plates was contrasted with that afforded by monolithic ultra-thin-layer chromatographic (UTLC) plates.
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